Antioxidant activity test of ethanol extract of red algae (Kappaphycus alvarezii) green variety from north coasts of Jepara district with DPPH method

Main Article Content

Arifah Irna Nur Widyastuti
Wulandari
Ahmad Fuad Masduqi

Abstract

Free radicals are unstable and highly reactive molecules that can cause cells damage. This cell damage can be inhibited by antioxidant compounds. One of the red algae, Kappaphycus alvarezii, is thought to contain phenolics and flavonoids compounds that can b used US antioxidants compounds. this study aims to determine the ethanol extract of red algae (Kappaphycus alvarezii) green variety has antioxidants activity using the DPPH method. this study also aims to determine the IC 50 value of the ethanolic extract of red algae (Kappaphycus alvarezii) green variety. The antioxidant activity test of red algae ethanol extract was carried out using the DPPH method. The results showed that the ethanolic extract of red algae (Kappaphycus alvarezii) green variety had antioxidant activity and the IC 50 value of the ethanolic extract of red algae was 242.28 ppm with a very weakcategory.

Article Details

How to Cite
Widyastuti, A. I. N., Wulandari, & Masduqi, A. F. (2023). Antioxidant activity test of ethanol extract of red algae (Kappaphycus alvarezii) green variety from north coasts of Jepara district with DPPH method. Journal of Health Management and Pharmacy Exploration, 1(1). https://doi.org/10.52465/johmpe.v1i1.133
Section
Articles

References

Hernani and M. Rahardjo, Tanaman Berkhasiat Antioksidan. Jakarta: Penebar Swadaya, 2006.

R. Darmawati and E. A. Jayadi, “Optimasi Pertumbuhan Caulerpa sp Yang Dibudidayakan Dengan Kedalaman Yang Berbeda di Perairan Laguruda Kabupaten Takalar,” Octopus, J. Ilmu Perikan., vol. 5, pp. 435–442, 2016. doi: 10.26618/octopus.v5i1.672. https://doi.org/10.26618/octopus.v5i1.672

R. Yanuarti, N. Nurjanah, E. Anwar, and T. Hidayat, “Profile of Phenolic and Antioxidants Activity from Seaweed Extract Turbinaria conoides and Eucheuma cottonii,” J. Pengolah. Has. Perikan. Indones., vol. 20, no. 2, p. 230, Aug. 2017, doi: 10.17844/jphpi.v20i2.17503. https://doi.org/10.17844/jphpi.v20i2.17503

A. Parenrengi and Sulaeman, “Mengenal Rumput Laut, Kappaphycus alvarezii,” Media Akuakultur, vol. 2, no. 1, pp. 142–146, 2007. doi: 10.15578/ma.2.1.2007.142-146. http://dx.doi.org/10.15578/ma.2.1.2007.142-146

Y. Ramdani, A. D. Ananto, and W. Hajrin, “Variasi Metode Ekstraksi dan Penetapan Nilai SPF Ekstrak Rumput Laut Merah (Eucheuma cottonii),” Acta Pharm. Indones. Acta Pharm Indo, vol. 9, no. 1, p. 31, Jun. 2021, doi: 10.20884/1.api.2021.9.1.4001. https://doi.org/ 10.20884/1.api.2021.9.1.4001

M. Akhlaghi and B. Bandy, “Mechanisms of flavonoid protection against myocardial ischemia–reperfusion injury,” J. Mol. Cell. Cardiol., vol. 46, no. 3, pp. 309–317, Mar. 2009, doi: 10.1016/j.yjmcc.2008.12.003. https://doi.org/10.1016/j.yjmcc.2008.12.003

G. Topçu, A. Ertaş, U. Kolak, M. Öztürk, and A. Ulubelen, “Antioxidant Activity Tests on Novel Triterpenoids from Salvia maacrochlamys,” ARKICOV, vol. 7, pp. 195–208, 2007. doi: 10.3998/ark.5550190.0008.716. http://dx.doi.org/10.3998/ark.5550190.0008.716

P. Molyneux, “The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity,” Songklanakarin J. Sci. Technol., vol. 26, no. December 2003, pp. 211–219, 2004, doi: 10.1287/isre.6.2.144.

F. Suhardinata and E. A. Murbawani, “Pengaruh Bubuk Daun Kenikir (Cosmos caudatus) Terhadap Kadar Malondialdehyde Plasma Tikus Wistar Diabetes Diinduksi Streptozotocin,” J. Nutr. Coll., vol. 4, no. 4, pp. 570–577, Oct. 2015, doi: 10.14710/jnc.v4i4.10164. https://doi.org/10.14710/jnc.v4i4.10164

Munira, Rasidah, and Marfina, “Uji Efektivitas Ekstrak Etanol Alga Merah (Eucheuma cottonii) Sebagai Anti Jamur Terhadap Candida albicans,” J. Kesehat. Ilm. Nasuwakes, vol. 8, pp. 1–7, 2015.

T. Robinson, Kandungan Organik Tumbuhan Tinggi. Bandung: ITB Press, 1995.

Departemen Kesehatan Republik Indonesia, Materia Medika Jilid VI, 1st ed. Jakarta: Depkes RI, 1995.

I. Kresnawaty and A. Zainuddin, “Aktivitas Antioksidan dan Antibakteri dari Derivat Metil Ekstrak Etanol Daun Gambir (Uncaria gambir),” J. Penelit. Tanam. Ind., vol. 15, no. 4, p. 145, Jun. 2020, doi: 10.21082/jlittri.v15n4.2009.145-151.

G. Marinova and V. Batchvarov, “Evaluation of The Methods for Determination of The Free Radical Scavenging Activity by DPPH,” Bulg. J. Agric. Sci., vol. 17, no. 1, pp. 11–24, 2011.

H. Bangngalino and M. Badai, “Analisis Kandungan Dan Aktivitas Antioksidan Pada Rumput Laut Eucheuma Cottoni Yang Diekstraksi Dengan Pelarut Etanol,” in Prosiding Seminar Hasil Penelitian (SNP2M), 2018, pp. 162–166.

P. L. Lantah, L. A. Montolalu, and A. R. Reo, “Kandungan Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Rumput Laut Kappaphycus alvarezii,” Media Teknol. Has. Perikan., vol. 5, no. 3, p. 73, 2017, doi: 10.35800/mthp.5.3.2017.16785. https://doi.org/10.35800/mthp.5.3.2017.16785

Abstract viewed = 273 times