Phytochemical contents and diuretic activity of ethanolic extract of the red leaf lettuce (Lactuca Sativa l.)
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Abstract
This study was conducted to evaluated of phytochemical content and diuretic activity of the red leaf lettuce. Red leaf lettuce were extracted by cold maceration method using 70% ethanol for the 3 days and remaceration for 1 days. The phytocemical content in ethanolic extract were evaluated by qualitatif method. A total of 25 male rats were divided into 5 groups with CMC Na, a standard drug (furosemide 10 mg/kg), and three different doses (200, 300, and 400 mg/kg) of ethanol extract. Parameters used to determine diuresis activity include first urine latency, urine pH and cumulative urine volume. The ethanolic extract induced diuresis in a dose dependent manner as compared to the negative control. Extracts at doses of 200, 300, and 400 mg/kg produced significant diuresis effects (p<0.05) compared to negative controls with values of diuretic action 1.35; 1.43; and 1.53, respectively. In addition, there was a slightly change in the pH of urine samples of the extract-treated group compared with the negative control. Phytochemicals analysis revealed the presence of alkaloids, flavonoids, phenolics, and tannins.
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