Antipyretic activity test of papaya leaf extract (Carica papaya L.) against peptone-induced male white mice
Article Sidebar
Main Article Content
Abstract
Fever increases body temperature above normal (98.6°F/ 37°C). To treat complaints of fever, antipyretic drugs are given. Papaya leaves are believed to have antipyretic effects. The purpose of this study was to test the antipyretic activity and effective dose of ethanol extract of papaya leaves (Carica papaya L.) against peptone-induced mice. The test animals were grouped into 5 groups consisting of group I (negative control) CMC Na 0.5%, group II (positive control) paracetamol drug 65 mg/KgBB mice, group III papaya leaf ethanol extract 140 mg/KgBW mice, group IV papaya leaf ethanol extract 280 mg/KgBW mice, and group V papaya leaf ethanol extract 560 mg/KgBW mice, with peptone fever inducer 10 mL/kgBW subcutaneously. The body temperature of the test animals was observed every 30 minutes for 240 minutes after peroral administration of the preparation, then obtained data on T0, Tdemam and body temperature measurements at each time. The data was then used to calculate the AUC and the average AUC calculation data was analyzed by Shapiro wilk test and One way Anova test. The results of the study of ethanol extract of papaya leaves have antipyretic effects, namely due to the presence of flavonoids, alkaloids, tannins, steroids, and saponins. Papaya leaf ethanol extract has the most effective antipyretic activity, namely a dose of 560 mg/kg BW, which is comparable to the positive control of paracetamol.
Article Details
References
R. Amili and Y. Lukmayani, “Uji efek antipiretik jus jeruk nipis pada tikus putih galur Sprague Dawley sel kelamin,” MIMBAR, vol. 24, no. 1, pp. 27–35, 2008.
N. Susanti, “Efektifitas Kompres Dingin Dan Hangat Pada Penataleksanaan Demam,” SAINSTIS, Apr. 2012, doi: 10.18860/sains.v0i0.1866.
P. Pushpangadan, V. George, T. Parambil Ijinu, and M. Ambika Chithra, “Biodiversity, Bioprospecting, Traditional Knowledge, Sustainable Development and Value Added Products: A Review,” J. Tradit. Med. Clin. Naturop., vol. 07, no. 01, 2018, doi: 10.4172/2573-4555.1000256.
J. A. Balogun, “Emerging Developments in Traditional Medicine Practice in Nigeria,” in The Nigerian Healthcare System: Pathway to Universal and High-Quality Health Care, Springer, 2022, pp. 235–275.
A. Ghaffar, B. Munir, M. Jahangeer, M. Ashiq, S. A. Qamar, and B. Ahmad, “Bioactivity prospection, antimicrobial, nutraceutical, and pharmacological potentialities of Carica papaya,” in Antiviral and Antimicrobial Smart Coatings, Elsevier, 2023, pp. 587–606.
T. Afsar, M. R. Khan, S. Razak, S. Ullah, and B. Mirza, “Antipyretic, anti-inflammatory and analgesic activity of Acacia hydaspica R. Parker and its phytochemical analysis,” BMC Complement. Altern. Med., vol. 15, no. 1, p. 136, Dec. 2015, doi: 10.1186/s12906-015-0658-8.
O. U. Estella et al., “Evaluation of the analgesic and antipyretic activity of methanol extract of Combretum bauchiense Hutch & Dalziel (Combretaceae) leaves,” Phytomedicine Plus, vol. 2, no. 1, p. 100166, Feb. 2022, doi: 10.1016/j.phyplu.2021.100166.
K. Jahnavi, P. Pavani Reddy, B. Vasudha, and B. Narender, “Non-steroidal anti-inflammatory drugs: an overview,” J. Drug Deliv. Ther., vol. 9, no. 1-s, pp. 442–448, Feb. 2019, doi: 10.22270/jddt.v9i1-s.2287.
N. Alim, “Aktivitas antipiretik ekstrak etanol daun beligo (Benincasa hispida (Thunb.) Cogn.) pada tikus,” J. Novem Med. Farm., vol. 1, no. 2, pp. 40–49, 2022, doi: https://doi.org/10.59638/junomefar.v1i2.610.
C. Lezin, P. Mauduit, G. Uzan, and M. E. Abdelgawad, “An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture,” Altern. to Lab. Anim., vol. 50, no. 5, pp. 339–348, Sep. 2022, doi: 10.1177/02611929221122780.
T. Belity et al., “Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses,” Int. J. Mol. Sci., vol. 23, no. 8, p. 4129, Apr. 2022, doi: 10.3390/ijms23084129.
C. Conidi, E. Drioli, and A. Cassano, “Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes,” Biomolecules, vol. 10, no. 6, p. 935, Jun. 2020, doi: 10.3390/biom10060935.