Antibacterial activity of flavonoid isolated from jamblang leaves (Syzigium Cumini (L.) Skeels) against Staphylococcus aureus

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Sania Nabila
Wulandari Wulandari
Endang Dwi Wulansari

Abstract

Jamblang leaves (Syzigium cumini (L.) Skeels) is one type plants used as traditional medicine. Jamblang leaves contain several phytochemical compounds such as alkaloids, flavonoids, resins, tannins, and oils essential. Flavonoid compounds contained in jamblang leaves have as antibacterial ability. The identification of flavonoid compounds in jamblang leaves and the antibacterial activity of flavonoid compounds against Staphylococcus aureus have been carried out. This study was conducted from jamblang leaves (Syzigium cumini (L.) Skeels) at concentrations of 2%, 4% and 6% against the growth of Staphylococcus aureus bacteria and to determine the type and structure of flavonoid compounds that have antibacterial activity. The method used for the extraction is remaceration, the separation of compounds using preparative thin layer chromatography while the antibacterial activity test using the pitting method and identification of flavonoid compounds using UV-Vis spectrophotometry. Extraction of 500 g of jamblang leaf powder using 96% ethanol resulted in a yield of 22,08%. The results of the study tested the antibacterial activity of flavonoid compounds of jamblang leaves obtained an average of clear zones produced at concentrations of 2%, 4% and 6% consecutively amounting to 0.9540 cm; 1.0540 cm; 1.1583 cm and ciprofloxacin 0.005 % by 1.4077%. Based on statistical analysis of the SPSS program with anava test 1 way obtained the results of significant activity differences between concentrations. Identification of the structure in UV-Vis spectrophotometry shows that flavonoid compounds lead to 3,7,4' trihydroxy flavonol.

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How to Cite
Nabila, S., Wulandari, W., & Wulansari, E. D. (2023). Antibacterial activity of flavonoid isolated from jamblang leaves (Syzigium Cumini (L.) Skeels) against Staphylococcus aureus. Journal of Health Management and Pharmacy Exploration, 1(2). https://doi.org/10.52465/johmpe.v1i2.187
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References

H. Arifin, N. Anggraini, D. Handayani, and R. Rasyid, “Standarisasi ekstrak etanol daun Eugenia cumini Merr,” J. Sains Tek. Far, vol. 11, no. 2, pp. 88–93, 2006.

A. Asmawati and J. Jumain, “Pengaruh Pemberian Ekstrak Daun Jamblang (Egenia cumini Merr.) Terhadap Pertumbuhan Streptococcus pyogenes,” Media Farm., vol. 16, no. 2, pp. 248–252, 2020.

A. Rahmadani, B. Budiyono, and S. Suhartono, “Gambaran keberadaan bakteri Staphylococcus aureus, kondisi lingkungan fisik, dan angka lempeng total di udara ruang rawat inap RSUD Prof. Dr. MA Hanafiah SM Batusangkar,” J. Kesehat. Masy., vol. 5, no. 5, pp. 492–501, 2017.

W. Bota, M. Martosupono, and F. S. Rondonuwu, “Potensi senyawa minyak sereh wangi (Citronella oil) dari tumbuhan Cymbopogon nardus L. sebagai agen antibakteri,” Pros. Semnastek, 2015.

D. A. Juwita, H. Arifin, and N. Yulianti, “Kajian Deskriptif Retrospektif Regimen Dosis Antibiotik Pasien Pneumonia Anak di RSUP. Dr. M. Djamil Padang,” J. Sains Farm. Klin., vol. 3, no. 2, p. 134, May 2017, doi: 10.29208/jsfk.2017.3.2.115.

R. H. Pratiwi, “Mekanisme pertahanan bakteri patogen terhadap antibiotik,” J. pro-life, vol. 4, no. 3, pp. 418–429, 2017.

B. Arifin and S. Ibrahim, “Struktur, Bioaktivitas Dan Antioksidan Flavonoid,” J. Zarah, vol. 6, no. 1, pp. 21–29, Apr. 2018, doi: 10.31629/zarah.v6i1.313.

A. Darmawati, I. Bawa, and I. W. Suirta, “Isolasi dan Identifikasi Senyawa Golongan Flavonoid pada Daun Nangka (Artocarpus heterophyllus Lmk) dan Aktivitas Antibakteri terhadap Bakteri Staphylococcus aureus,” J. Kim., vol. 9, no. 2, pp. 203–210, 2015.

D. K. RI, “Materia Medika Indonesia (Jilid II).” Depkes RI, 1995.

Y. P. Utami, I. Imrawati, and A. Rasyid, “Isolasi dan Karakterisasi Ekstrak Etanol Daun Leilem (Clerodendrum Minahassae Teijsm Dan Binn.) Dengan Metode Spektrofotometri Isolation And Characterization Ethanol Extract Of Leilem Leaves (Clerodendrum Minahassae Teijsm And Binn) With Methods Spectrophotometric,” J. Farm. Medica/Pharmacy Med. J., vol. 1, no. 2, 2018.

K. R. Markham, “Cara mengidentifikasi flavonoid,” Bandung Itb, 1988.

Mutiara E,V and Wildan Achmad, " Ekstraksi Flavonoid Dari Daun Pare (Momordica Charantia L.) Berbantu Gelombang Mikro Sebagai Penurun Kadar Glukosa Secara In Vitro. Metana, Vol. 10 No. 01, Juli 2014, pp. 1-11

K. Elvira, E. Fachriyah, and D. Kusrini, “Isolation of Flavonoid Compounds from Eceng Gondok (Eichhornia crassipes) and Antioxidant Tests with DPPH (1, 1-Diphenyl-2-Picrylhydrazyl) Method,” J. Kim. Sains dan Apl., vol. 21, no. 4, pp. 187–192, 2018

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